Afla B Aflatoxin Testing System

Aflatoxin Afla B Affinity Column

Aflatoxin, a toxin from a naturally occurring mold, is a Group 1 carcinogen, proven to cause cancer in humans. Aflatoxin is made up of the sum of 4 total aflatoxins: B1, B2, G1, and G2 ; however, in some cases only B1 is regulated. For example, within the European Community B1 is regulated at the 2ppb level.

Afla B™ from VICAM is the trusted aflatoxin B test that produces precise numerical results. Using monoclonal antibody-based affinity chromatography, Afla B can isolate aflatoxins B1 and B2 from feeds, food, grains, and nuts via fluorometric or HPLC detection.

Whereas aflotoxin B1 can be singled out and measured uniquely by HPLC, in fluorometric measurements, total B1 and B2 is reported. Then it is known that B1 accounts for 94% of total B1 and B2 asmeasured in corn and for 82.5% asmeasured in peanuts1.

Afla B is safe and simple. It can be performed in less than 10 minutes and requires no special skills. With Afla B, you have the best of all worlds: sensitivity, simplicity, and speed – quick tests for parts per billion or parts per trillion levels. In fact, no other test comes close for speed, quantification, and economy.

1. Data supplied by D.M.Wilson (unpublished), Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, Tifton, GA 31793, USA

Download a Datasheet for this Product (PDF)

Benefits

  • Convenient – For use with fluorometer or HPLC
  • Durable – Long shelf life; requires no refrigeration
  • Versatile – For use with a variety of samples
  • Quick – Less than 10 minutes to isolate toxin*
  • Wide Range – Detects levels as low as 1 ppb
    (fluorometer) and 50 ppt (HPLC) and as high as 300 ppb
  • Safe – Requires less toxicmaterials than other methods

* excludes preparation and extraction

Here’s How Simple
AFLA B is to Use:

Extract Sample
Grind and weigh sample
Add salt and methanol/water mixture
Filter
Dilute and Filter
Dilute extract
Filter
Absorb and Elute
Pass filtrate over Afla B affinity column
Wash column with water
Elute aflatoxins with methanol and collect in a cuvette
Measure
Add developer and place cuvette into a calibrated fluorometer, or
Inject eluate into HPLC
Determine aflatoxin concentration

Ordering Information

Cat. No. Description
G1003  Afla B™ Columns (25 per box)
G1004  Afla B Columns (50 per box)
G1124  Noniodized Sodium Chloride (salt, NaCl)
G8000  Series-4EX Fluorometer™
G8001  AflaTest® Basic Equipment Package 110 V
G8013  AflaTest Basic Equipment Package 220 V
20050  Graduated Cylinder, 50 mL
20100  Digital Scale with AC Adapter
20200  Commercial Blender with Stainless Steel Container
20250  Graduated Cylinder, 250 mL
20501  500 mL Bottle Dispenser for Methanol (0 – 3 mL range)
20600  50 mL Bottle Dispenser for Developer (0 – 3 mL range)
20652  Disposable Plastic Pipets, 1 mL (50)
20700  Wash Bottle, 500 mL
21010  Cuvette Rack
Cat. No. Description
21020  Single Position Pump Stand †
23040  Vortex Mixer
31240  VICAM Fluted Filter Paper, 24 cm (100)
31955  Microfiber Filters, 1.5 μm, 11 cm (100)
31967  Kim Wipes
32010  AflaTest Developer (50 mL)
33020  Mycotoxin Calibration Standards
34000  Disposable Cuvettes (250)
35016  Methanol, HPLC Grade (4 x 4L)
36010  Disposable Plastic Beakers (25)
36020  Filter Funnel, 65 mm (10)
36222  Filter Funnel, 105 mm (4)
 
Also required;must be sourced locally:
Distilled, Reverse Osmosis, or Deionized Water

†Multiple position pump stands available

Subject to change without notice. ©1998, 2002, 2007 VICAM. Afla B™ is a trademark of VICAM. All VICAM products are protected by worldwide patents and trademarks.

VICAM, a Waters Business
34 MAPLE ST.
MILFORD, MA 01757
TEL: 800-338-4381, 508-482-4935
FAX: 508-482-4972
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with Afla B™ products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the Afla B procedures.

The user assumes all risk in using Afla B analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that Afla B products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of mechantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or Afla B products.

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