VICAM

• Mycotoxin

AflaOchra HPLC Aflatoxin Testing System

Aflatoxin, a toxin froma naturally occurringmold, is a Group 1 carcinogen proven to cause cancer in humans. Throughout the world, there are significant regulations on the presence of aflatoxins in food. The European Commission has action levels for aflatoxins in groundnuts, nuts, dried fruit, and cereals intended for direct human consumption or for ingredients in foodstuffs. These action levels are 4 ppb total aflatoxins and 2 ppb for aflatoxin B₁.

Ochratoxin A is amycotoxin produced by the fungus Aspergillus ochraceous and also by several species of Penicillium. Ochratoxin A has been known to cause kidney damage in animals and is a suspected carcinogen.

AflaOchra HPLC™ from VICAM is the only test that employs a single column to produce precise numerical results in determining both aflatoxin and ochratoxin levels. Using monoclonal antibody based affinity chromatography, AflaOchra HPLC can isolate aflatoxins B₁, B₂, G₁ and G₂ from grain, food, feed, and nuts and ochratoxin A and its stereoisomer from coffee, beer, grain, and feed. With AflaOchra HPLC you have the best of all worlds: sensitivity, simplicity, and speed—quick tests with results in parts per billion. In fact, no other test comes close for speed, quantification, and economy.

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Benefits Exclusive – For HPLC use only Comprehensive – Detects aflatoxins B1, B2, G1 and G2, ochratoxin A and it’s stereoisomer Durable – Long shelf life; requires no refrigeration Versatile – Can be used with a variety of samples Quick – Less than 10 minutes to isolate toxins* Wide Range – Measures levels as low as 0.25 ppb and as high as 100 ppb Safe – Requires less toxic materials than other methods *excludes preparation and extraction

Here’s How Simple AflaOchra HPLC is to Use: Extract Sample Grind and weigh sample Add salt andmethanol/water mixture Filter Dilute and Filter Dilute extract Filter Absorb and Elute Pass filtrate over AflaOchra HPLC affinity column Wash column Elute aflatoxins and ochratoxin A and collect in cuvette Measure Inject eluate into HPLC and determine aflatoxins and ochratoxin A concentrations

Ordering Information

Cat. No. Description G1017  AflaOchra HPLC™ Columns (25 per box) G1118  WB Column Coupling G1124 Noniodized Sodium Chloride (Salt, NaCl) G1130  Acetonitrile (4 x 4 L) G4033 Micro-pipettor, 1 mL 20050  Graduated Cylinder, 50 mL 20100  Digital Scale with AC Adapter 20200  Commercial Blender with Stainless Steel Container 20250  Graduated Cylinder, 250 mL 20300  Glass Blender Jar 20501  500 mL Bottle Dispenser for Methanol (0-3 mL range) 20656  Micro-pipette Tips for 1 mL Micropipettor (100) 20700  Wash Bottle, 500 mL 21010  Cuvette Rack 21020  Single Position Pump Stand†

Cat. No. Description 23040  Vortex Mixer 31240  VICAM Fluted Filter Paper, 24 cm (100) 31955  Microfiber Filters, 1.5 μm, 11 cm (100) 31967  Kim Wipes 34000  Disposable Cuvettes (250 per pack) 34010  Glass Syringe, 10 mL 35016  Methanol, HPLC Grade (4 x 4 L) 36010  Disposable Plastic Beakers (25 per pack) 36020  Filter Funnel, 65 mm (10 per pack) 36022  Filter Funnel, 105 mm (4 per pack)   Also required; must be sourced locally: Distilled, Reverse Osmosis, or Deionized Water †Multiple position pump stands available

Subject to change without notice. ©1999, 2002, 2007 VICAM. AflaOchra HPLC™ is a trademark of VICAM. All VICAM products are protected by worldwide patents and trademarks.

VICAM, a Waters Business
34 MAPLE ST.
MILFORD, MA 01757
TEL: 800-338-4381, 508-482-4935
FAX: 508-482-4972
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with AflaOchra HPLC™ products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the AflaOchra HPLC procedures.

The user assumes all risk in using AflaOchra HPLC analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that AflaOchra HPLC products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or AflaOchra HPLC products.

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©2001, VICAM