VICAM

• Mycotoxin

AflaTest Mycotoxin Testing System

Aflatoxin, a toxin from a naturally occurring mold, is a Group 1 carcinogen proven to cause cancer in humans.

The United States federal government action level for aflatoxin in food for human consumption or in dairy cowfeed is 20 ppb. For milk for human consumption the level is 0.5 ppb. Levels up to 300 ppb are allowed in certain circumstances for nondairy animal feed.

AflaTest® from VICAM is the trusted aflatoxin test that produces precise numerical results. Using monoclonal antibody-based affinity chromatography, AflaTest can isolate aflatoxins B₁, B₂, G₁, and G₂ from feeds, food, grains, and nuts, and M₁ from dairy products.

AOAC and FGIS approved, AflaTest is safe and simple. It can be performed in less than 10 minutes and requires no special skills. Results may be recorded using a digital fluorometer readout or automatic printing devices. AflaTest is also ideal as the cleanup step for any HPLC analysis. With AflaTest, you have the best of all worlds: sensitivity, simplicity, and speed—quick tests for parts per billion levels. In fact, no other test comes close for speed, quantification, and economy.

Download a Datasheet for this Product (PDF)

Benefits Convenient – For use with fluorometer or HPLC Comprehensive – Total readings for all aflatoxins Durable – Long shelf life; requires no refrigeration Versatile – For use with a variety of samples Quick – Less than 10 minutes to isolate toxin* Wide Range – Detects levels as high as 300 ppb and as low as 0.1 ppb** Safe – Requires less toxic materials than other methods *excludes preparation and extraction ** 1 ppb for grain and nuts

Here’s How Simple Aflatest is to Use: Extract Sample Grind and weigh sample Add salt and methanol/water mixture Filter Dilute and Filter Dilute extract Filter Absorb and Elute Pass filtrate over AflaTest affinity column Wash column Elute aflatoxins with methanol and collect in cuvette Measure Add developer and place cuvette into a calibrated fluorometer, or Inject eluate into HPLC Determine aflatoxin concentration

Ordering Information

Cat. No. Description G8001  AflaTest® Basic Equipment Package 110 V G8004  AflaTest Peanut Basic Equipment Package, 110 V G8013  AflaTest Basic Equipment Package 220 V G1010  AflaTest Columns (25 per box) 12022  AflaTest Columns (50 per box) G1124  Noniodized Sodium Chloride (salt, NaCl) G2007  0.22 mm Nylon Membrane Syringe Filters (50 per pack) G4033  Micro-pipettor, 1 mL G8000  Series-4EX Fluorometer™ 20050  Graduated Cylinder, 50 mL 20100  Digital Scale with AC Adapter 20200  Commercial Blender with Stainless Steel Container 20250  Graduated Cylinder, 250 mL 20300  Glass Blender Jar 20501  500 mL Bottle Dispenser for Methanol (0-3 mL range) 20600  50 mL Bottle Dispenser for Developer (0-3 mL range) 20652  Disposable Plastic Pipets, 1 mL (50 per pack) 20656  Micro-pipette Tips for 1mLMicro-pipettor (100) 20700  Wash Bottle, 500 mL

Cat. No. Description 21010  Cuvette Rack 21020  Single Position Pump Stand† 23040  Vortex Mixer 31240  VICAM Fluted Filter Paper, 24 cm (100) 31955  Microfiber Filters, 1.5 μm, 11 cm (100) 31967  Kim Wipes 32010  AflaTest Developer (50 mL) 33020  Mycotoxin Calibration Standards 33030  AflaTest-FGIS Calibration Standards 33040  AflaTest-M Calibration Standards 33501  Tween 20 (50 mL) 34000  Disposable Cuvettes (250 per pack) 35016  Methanol, HPLC Grade (4 x 4 L) 36010  Disposable Plastic Beakers (25 per pack) 36020  Filter Funnel, 65 mm (10 per pack) 36222  Filter Funnel, 105 mm (4 per pack)   Also required; must be sourced locally: Centrifuge capable of obtaining 2000 x g RCG* Distilled, Reverse Osmosis, or Deionized Water   †Multiple position pump stands available * Required only for testing of milk

Subject to change without notice.
©1998, 2002, 2007 VICAM. AflaTest® is a trademark of VICAM.
All VICAM products are protected by worldwide patents and trademarks.

VICAM, a Waters Business
34 MAPLE ST.
MILFORD, MA 01757
TEL: 800-338-4381, 508-482-4935
FAX: 508-482-4972
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with AflaTest® products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the AflaTest procedures.

The user assumes all risk in using AflaTest analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that AflaTest products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or AflaTest products.

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