CitriTest HPLC Mycotoxin Testing System

CitriTest Affinity Column

Citrinin, a toxin from a naturally occurring mold, contaminates rice, wheat, flour, barley, maize, rye, oats, peanuts, and fruit. It is also a widespread contaminant of red rice, which is not only a dietary staple in many Asian countries, but also a main ingredient in many other food products. It is also used in the popular herbal supplement red yeast rice extract which is used to reduce cholesterol levels.

Produced by Aspergillus ochraceus and Penicillium citrinum, citrinin
has been known to cause kidney damage and mild liver damage in livestock and is suspected to cause kidney and liver damage in humans. Because of concerns over citrinin contamination, Japan has issued an advisory limit of 200 ppb and the European Union has a recommended limit of 100 ppb.

Using monoclonal antibody-based affinity chromatography, CitriTest™ HPLC is a new HPLC-only test for the detection of citrinin using immunoaffinity columns. Performed in less than 10 minutes, CitriTest HPLC can measure levels of citrinin as low as 10 ppb and as high as 500 ppb.

An ideal cleanup step for any HPLC, CitriTest HPLC has a long shelf life and provides precise numerical results in parts per bllion levels. The preferred test of laboratories around the world, no other test comes close for speed, quantification, and specificity.

Benefits

  • Exclusive — Specifically for HPLC use
  • Convenient — Uses same methods as other VICAM tests
  • Durable — Long shelf life
  • Quick — 15 minutes to isolate toxin*
  • Wide Range — Detects levels as low as 10 ppb and
    as high as 500 ppb

*excluding preparation and extraction

Here’s How Simple
CitriTest HPLC is to Use:

Extract Sample
Grind and weigh sample
Add methanol/water mixture
Filter
Dilute and Filter
Dilute extract
Absorb and Elute
Pass filtrate over CitriTest HPLC affinity column
Wash column
Elute citrinin and collect in cuvette
Measure
Inject into HPLC
Determine citrinin concentration

Ordering Information

Cat. No. Description
G1070  CitriTest™HPLC Columns (25 per box)
G1118  WB Column Coupling (6)
G1130  Acetonitrile (4 x 4 L)
G4033  Micro-pipettor, 1 mL
20050  Graduated Cylinder, 50 mL
20100  Digital Scale with AC Adapter
20200  Commercial Blender with Stainless Steel Container
20250  Graduated Cylinder, 250 mL
20656  Micro-pipette Tips for 1 mL Micro-pipettor (100)
21010  Cuvette Rack
21020  Single Position Pump Stand†
Cat. No. Description
23040  Vortex Mixer
31240  VICAM Fluted Filter Paper, 24 cm (100)
31955  Microfiber Filters, 1.5μm, 11 cm (100)
34000  Disposable Cuvettes (250 per pack)
35016  Methanol, HPLC Grade (4 x 4 L)
36010  Disposable Plastic Beakers (25 per pack)
36020  Filter Funnel, 65 mm (10 per pack)
36022  Filter Funnels, 105 mm (4 per pack)
 
Also required; must be sourced locally:
O-phosphoric acid, HPLC grade
Distilled, Reverse Osmosis, or Deionized Water
 
†Multiple position pump stands available

Subject to change without notice.
©2007 VICAM. CitriTest™HPLC is a trademark of VICAM.
All VICAM products are protected by worldwide patents and trademarks.

VICAM
313 PLEASANT STREET
WATERTOWN, MA 02472
TEL: 800.338.4381, 617.926.7045
FAX: 617.923.8055
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with CitriTest™HPLC products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the CitriTest HPLC procedures.

The user assumes all risk in using CitriTest HPLC analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that CitriTest HPLC products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or CitriTest HPLC products.

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