DONtest FL+ Mycotoxin Testing System

DONtest FL+ Affinity Column

Deoxynivalenol (DON) is also known as vomitoxin because of its impact on livestock through interference with animal growth and acceptance of feed. DON is often present with other mycotoxins and has been isolated from grains and feeds throughout the world at levels as high as 92 ppm. DON has been implicated in moldy corn toxicosis of swine and may play a role in the human disease alimentary toxic aleukia. Because of concerns about DON, many customers require levels of less than 2.0 ppm for animal feed and 1.0 ppm for commodities destined for human consumption. The United States Food and Drug Administration (FDA) advises less than 1ppm DON in finished wheat products for human consumption with levels of 5ppm and 10ppm advised for animal feeds.

DONtest™FL+ from VICAM's a rapid mycotoxin test which produces precise numerical results. Using monoclonal antibody-based affinity chromatography, DONtest FL+ can isolate DON with a method that is reliable, rapid, and works well under field conditions.

DONtest FL+ is safe and simple. Like our other columns, it can be performed rapidly and requires no special skills. You have the best of all worlds: speed, sensitivity, and simplicity – quick tests for parts per million using a fluorometer.

Benefits

  • Exclusive — Specifically for fluorometer use
  • Durable — Long shelf life
  • Wide Range — Detects levels as low as 0.50 ppm and as high as 5 ppm
  • Safe — Requires less toxic materials than other methods

Here’s How Simple
DONtest WB is to Use:

Extract Sample
Grind and weigh sample
Blend sample with salt and water
Filter
Absorb and Elute
Pass filtrate over DONtest FL+ affinity column
Wash column with water
Elute DON fromcolumn and collect in a cuvette
Measure
Add developers and place cuvette into a calibrated fluorometer
Determine DON concentration

Ordering Information

Cat. No. Description
G1048 DONtest FL+ Columns (50/box)
G1049  DONtest FL+ Columns (25/box)
G1118  WB Column Coupling (6)
G1124  Noniodized Sodium Chloride (Salt, NaCl)
G2000  DONtest Cuvettes (250 per pack)
G2012  Micro-pipette Tips for 20 μL - 200 μL
Adjustable Micro-pipettor
G2013  Micro-pipette Tips for 1 mL Adjustable Micro-pipettor
G2014  60 mL Amber Glass Bottles (2)
G4047  Graduated Cylinder, 10 mL
G4077  Dry Heating Block
G4078  Dry Heating Block Thermometer
G4079  20 μL - 200 μL Adjustable Micro-pipettor
G4080  1 mL Adjustable Micro-pipettor
G5015  DONtest FL+ Developer A
G5016  DONtest FL+ Developer B
G8000  Series-4EX Fluorometer™
20100  Digital Scale with AC Adapter
20200  Commercial Blender with Stainless Steel
Container
Cat. No. Description
20250  Graduated Cylinder, 250 mL
20657  Disposable Plastic Pipets, 1.5 mL (50)
20700  Wash Bottle, 500 mL
21010  Cuvette Rack
21020  Single-Position Pump Stand with Hand Pump
23040  Vortex Mixer
31242  DONtest Fluted Filter Paper, 24 cm (100)
31955  Microfiber Filters, 1.5 μm, 11 μm (100)
31967  Kim Wipes
33020  Mycotoxin Calibration Standards
35016  Methanol, HPLC Grade (4 x 4L)
 
Also required; must be sourced locally:
Compressed Air
Purified Water

Subject to change without notice.
©2006 VICAM. DONtest FL+ is a trademark of VICAM.
All VICAM products are protected by worldwide patents and trademarks.

VICAM
313 PLEASANT STREET
WATERTOWN, MA 02472
TEL: 800-338-4381, 617-926-7045
FAX: 617-923-8055
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with DONtest™ FL+ products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the DONtest FL+ procedures.

The user assumes all risk in using DONtest FL+ analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that DONtest FL+ products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or DONtest FL+ products.

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