DONtest HPLC Vomitoxin Testing System from VICAM

DONtest HPLC Vomitoxin Testing System

Deoxynivalenol (DON) is also known as vomitoxin because of its impact on livestock through interference with animal growth and acceptance of feed. DON is often present with other mycotoxins and has been isolated from grains and feeds throughout the world at levels as high as 92 ppm. DON has been implicated in moldy corn toxicosis of swine and may play a role in the human disease alimentary toxic aleukia. Because of concerns about DON, the United States FDA has issued advisory levels of 1 ppm for wheat products for human consumption, 5 ppmfor grain products for most animal feeds and 10 ppm for grain products for cattle feed. The European Union has limits for DON that range from less than 0.2 ppm in processed cereal-based foods for infants and young children to less than 1.75 ppm for unprocessed wheat.

Using monoclonal antibody-based affinity chromatography, DONtest™ HPLC is an HPLC-only test for the detection of DON using immunoaffinity columns. Performed in less than 10 minutes, DONtest HPLC can measure levels of DON as low as 0.10 ppm and as high as 5 ppm.

An ideal cleanup step for any HPLC, DONtest HPLC has a long shelf life and provides precise numerical results in parts permillion levels. The preferred test of laboratories around the world, no other test comes close for speed, quantification, and specificity.

Benefits

Exclusive – Specifically for HPLC use
Convenient – Uses same methods as other VICAM tests
Durable – Long shelf life
Versatile – Can be used with a variety of samples
Wide Range – Detects levels as low as 0.10 ppm and as high as 5 ppm
Safe – Requires less toxic materials than other methods

Here’s How Simple
DONtest HPLC is to Use:

Extract Sample: Grind and weigh sample; Add PEG and water to sample; Filter
Absorb and Elute: Pass filtrate over DONtest HPLC affinity column; Wash column; Elute DON and collect in cuvette
Measure: Dry down eluate; Resuspend in mobile phase; Inject into HPLC; Determine DON concentration

Ordering Information

Cat. No.	Description
G1005	DONtest HPLC Columns (25)
G1006	DONtest HPLC Columns (50)
G1015	Polyethylene Glycol (PEG), 250g
G1130	Acetonitrile (4 x 4 L)
G4033	Micro-pipettor, 1 mL
20100	Digital Scale with AC Adapter
20200	Commercial Blender w/Stainless Steel Container
20250	Graduated Cylinder, 250 mL
20300	Glass Blender Jar, 500 mL
20501	500 mL Bottle Dispenser for Methanol (0-3 mL range)
20656	Micro-pipette Tips for 1 mL Micro-pipettor (100)

Cat. No.	Description
20700	Wash Bottle, 500 mL
21020	Single Position Pump Stand†
23040	Vortex Mixer
31242	DONtest Fluted Filter Paper for HPLC, 24 cm (100)
31955	Microfiber Filters, 1.5 μm, 11 cm (100)
31967	KimWipes
34000	Disposable Cuvettes (250 per pack)
34010	Glass Syringe Barrel, 10 mL
35016	Methanol, HPLC Grade (4 x 4 L)
36010	Disposable Plastic Beakers (25 per pack)
36020	Filter Funnel, 65 mm (10 per pack)
36022	Filter Funnel, 105 mm (4 per pack)
Also required; must be sourced locally: Distilled, Reverse Osmosis, or Deionized Water Speedvac or Equivalent Evaporator †Multiple position pump stands available

VICAM
313 PLEASANT STREET
WATERTOWN, MA 02472
TEL: 800-338-4381, 617-926-7045
FAX: 617-923-8055
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with DONtest™ HPLC products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the DONtest HPLC procedures.

The user assumes all risk in using DONtest HPLC analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that DONtest HPLC products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or DONtest HPLC products.

VICAM