DONtest WB Vomitoxin Testing System

DONtest WB Affinity Column

Deoxynivalenol (DON) is also known as vomitoxin because of its impact on livestock through interference with animal growth and acceptance of feed. DON is often present with other mycotoxins and has been isolated from grains and feeds throughout the world at levels as high as 92 ppm. DON has been implicated in moldy corn toxicosis of swine and may play a role in the human disease alimentary toxic aleukia. Because of concerns about DON, many customers require levels of less than 2.0 ppm for animal feed and 1.0 ppm for commodities destined for human consumption. The United States FDA has instituted advisory levels of 1 ppm for wheat products for human consumption, 5 ppm for grain products for most animal feeds and 10 ppm for grain products for cattle feed. The European Community has regulations for DON levels that range from less than 0.2 ppm in processed cereal-based foods for infants and young children to less than 1.75 ppm for unprocessed wheat.

Using monoclonal antibody-based affinity chromatography, DONtest™WB is a new HPLC-only test for the detection of DON using wide bore immunoaffinity columns. With a total volume of 3 mL, compared to the total of 1mL in VICAM’s regular columns, DONtest WB allows for a faster flow rate preferred by many laboratories and is the ideal cleanup step for any HPLC.

DONtest WB is one of many wide bore columns available from VICAM. Through the development of this fast-flow WB product line, VICAM demonstrates a commitment to improving mycotoxin testing and responding to our customers needs.

Benefits

  • Exclusive — Specifically for HPLC use
  • Durable — Long shelf life
  • Versatile — For use with a variety of samples
  • Quick — 10 minutes to isolate toxin*
  • Wide Range — Detects levels as low as 0.04 ppm and
    as high as 5 ppm
  • Fast Flow — Faster results than narrow bore test

*excluding preparation and extraction

Here’s How Simple
DONtest WB is to Use:

Extract Sample
Grind and weigh sample
Add water and shake
Filter
Absorb and Elute
Pass filtrate over DONtest WB affinity column
Wash column
Elute DON and collect in a cuvette
Dry eluate and reconstitute
Measure
Inject eluate into HPLC
Determine DON concentration

Ordering Information

Cat. No. Description
G1065  DONtest™WB Columns (25 per box)
G1066  DONtest WB Columns (50 per box)
G1130  Acetonitrile (4 x 4 L)
G4033  Micro-pipettor, 1 mL
20100  Digital Scale with AC Adapter
20250  Graduated Cylinder, 250 mL
20501  500 mL Bottle Dispenser for Methanol (0-3 mL range)
20656  Micro-pipette Tips for 1mL Micro-pipettor (100)
20700  Wash Bottle, 500 mL
21010  Cuvette Rack
21020  Single Position Pump Stand†
23040  Vortex Mixer
Cat. No. Description
31240  VICAM Fluted Filter Paper, 24 cm (100)
31955  Microfiber Filters, 1.5 μm, 11 cm (100)
31967  Kim Wipes
34000  Disposable Cuvettes (250 per pack)
35016  Methanol, HPLC Grade (4 x 4 L)
36010  Disposable Plastic Beakers (25 per pack)
36020  Filter Funnel, 65 mm (10 per pack)
36022  Filter Funnel, 105 mm (4 per pack)
 
Also required; must be sourced locally:
Mechanical Shaker
Purified Water
Speedvac or Equivalent Evaporator
 
†Multiple position pump stands available

Subject to change without notice.
©2007 VICAM. DONtest™WB is a trademark of VICAM.
All VICAM products are protected by worldwide patents and trademarks.

VICAM
313 PLEASANT STREET
WATERTOWN, MA 02472
TEL: 800-338-4381, 617-926-7045
FAX: 617-923-8055
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with DONtest™WB products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the DONtest WB procedures.

The user assumes all risk in using DONtest WB analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that DONtest WB products conform to VICAM’s printed specification and quality control standards. VICAMwill, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or DONtest WB products.

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