VICAM

• Mycotoxin
• Microbiological

FumoniTest Fumonisin Testing System

Fumonisins are mycotoxins produced by the fungus Fusarium moniliforme. Frequent, almost universal inhabitants of corn, Fumonisin B₁ and fumonisin B₂ are present in most corn samples tested, often at greater than 1 ppm. The FDA/USDA Working Group on Fumonisin advises less than 5 ppm in horse feed, less than 10 ppm for swine feed and less than 50 ppm for cattle feed. Fumonisin is thought to cause equine leukoencephalomalacia in horses, swine pulmonary edema, and human esophageal cancer.

Using monoclonal antibody-based affinity chromatography, FumoniTest™ from VICAM is the trusted fumonisin test that produces precise numerical results.

FumoniTest is safe and simple. It can be performed in less than 15 minutes and requires no special skills. FumoniTest is also ideal as the cleanup step for any HPLC analysis for precise results in parts per million. With FumoniTest you have the best of all worlds: sensitivity, simplicity, and speed—quick tests for parts per million levels.

FumoniTest has a long shelf life. In fact, no other test comes close for speed, quantification, and economy

Benefits Convenient – For use with fluorometer or HPLC Comprehensive – Tests for fumonisins B1, B2, and B3 Durable – Has a long shelf life Versatile – For use with a variety of samples Quick – Less than 15 minutes to isolate toxin* Wide Range – Detects levels as low as 0.016 ppm and as high as 10 ppm * excludes preparation and extraction

Here’s How Simple DONtest HPLC is to Use: Extract Sample Grind and weigh sample Add salt and methanol/ water mixture Filter Dilute and Filter Dilute extract Filter Absorb and Elute Pass filtrate over FumoniTest affinity column Wash column with buffers Elute fumonisins and collect in cuvette Measure Add developers and place cuvette into a calibrated fluorometer, or Inject eluate into HPLC Determine fumonisin concentration

Ordering Information

Cat. No. Description G8008 FumoniTest™ Basic Equipment Package 110V G8009  FumoniTest Basic Equipment Package 220V G1008  FumoniTest Columns (25 per box) G1112  10x Concentrate of 0.1% Tween PBS (150 mL) G1113  10x Concentrate of PBS (150 mL) G1124  Noniodized Sodium Chloride (Salt, NaCl) G2005  Microfiber Filters, 1.0 μm, 9 cm (100) G4031  Micro-pipettor, 20 μL G4033  Micro-pipettor, 1 mL G5003  FumoniTest Developer A HPLC (5 mL) G5004  FumoniTest Developer B HPLC/Fluorometer (500 μL) G5005  FumoniTest Developer A Fluorometer (15 mL) G8000  Series 4-EX Fluorometer™ 20050  Graduated Cylinder, 50 mL 20100  Digital Scale with AC Adapter 20200  Commercial Blender with Stainless Steel Container

Cat. No. Description 20250  Graduated Cylinder, 250 mL 20501  500 mL Bottle Dispenser for Methanol (0-3 mL range) 20652  Disposable Plastic Pipets (50 per pack) 20656  Micro-pipette Tips for 1 mL Micro-pipettor (100) 20658  Micro-pipette Tips for 20 μL Micro-pipettor (96) 20700  Wash Bottle, 500 mL 21010  Cuvette Rack 21020  Single Position Pump Stand† 23040  Vortex Mixer 31240  VICAM Fluted Filter Paper, 24 cm (100) 31967  Kim Wipes 31955  Microfiber Filters, 1.5 μm, 11 cm (100) 33060  FumoniTest Calibration Standards 34000  Disposable Cuvettes (250 per pack) 35016  Methanol, HPLC Grade (4 x4 L) 36010  Disposable Plastic Beakers (25 per pack) 36020  Filter Funnel, 65 mm (10 per pack) 36022  Filter Funnel, 105 mm (4 per pack)   Also required; must be sourced locally: Distilled, Reverse Osmosis, or Deionized Water Sodium Bicarbonate (for testing beer only) Speed Vacuum or Equivalent Evaporator   †Multiple position pump stands available

Subject to change without notice. ©1998, 2004, 2007 VICAM. FumoniTest™ is a trademark of VICAM. All VICAM products are protected by worldwide patents and trademarks.

VICAM
313 PLEASANT STREET
WATERTOWN, MA 02472
TEL: 800-338-4381, 617-926-7045
FAX: 617-923-8055
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with FumoniTest™ products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the FumoniTest procedures.

The user assumes all risk in using FumoniTest analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that FumoniTest products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship ormaterial. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or FumoniTest products.

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