VICAM

• Mycotoxin
• Microbiological

OchraTest WB Ochratoxin Testing System

Ochratoxin A is amycotoxin produced by the fungus Aspergillus ochraceous and also by several species of Penicillium fungi. Frequently found in green coffee, ochratoxin A will remain present even after the roasting process.

Ochratoxin A (OTA) is also commonly found in wine, baby food, raw and processed cereals, and dried vine fruits–all of which are regulated by European limits as low as 0.5 ppb. Known to cause kidney damage in animals and as a carcinogen, OTA finds its way into human and animal diets not only from EU-regulated commodities, but also frombarley, oats, and sorghum.

Using monoclonal antibody-based affinity chromatography, OchraTest™WB is a new HPLC-only test for the detection of ochratoxin A using wide bore immunoaffinity columns.With a total volume of 3mL, compared to the total of 1mL in VICAM’s standard columns, OchraTest WB allows for a faster flow rate preferred bymany laboratories and is the ideal cleanup step for any HPLC.

OchraTest WB is one of many wide bore columns available from VICAM. Through the development of this fast-flow WB product line, VICAM demonstrates a commitment to improving mycotoxin testing and responding to our customers’ needs.

Benefits Exclusive – Specifically for HPLC use Durable – Long shelf life; requires no refrigeration Versatile – For use with a variety of samples Quick –10 minutes to isolate toxin* Wide Range – Detects levels as low as .25 ppb and as high as 100 ppb Fast Flow – Passes more volume over the column *excluding preparation and extraction

Here’s How Simple Aflatest WB is to Use: Extract Sample Grind and weigh sample Add salt and methanol/water mixture Filter Dilute and Filter Dilute extract Filter Absorb and Elute Pass filtrate over OchraTest WB affinity column Wash column Elute ochratoxin A and collect in a cuvette Measure Dilute and inject eluate into HPLC Determine ochratoxin A concentration

Ordering Information

Cat. No. Description G1033 OchraTest WB Columns (25 per Box) G1034  OchraTest WB Columns (50 per Box) 31240  VICAM Fluted Filter Paper, 24 cm (100) 31955  Microfiber Filters, 1.5 μm, 11 cm (100) 34000  Disposable Cuvettes (250 per Pack) 35016  Methanol, HPLC Grade (4 X 4 L) 36010  Disposable Plastic Beakers (25 per pack) G1113  10X concentrate of PBS (150 mL) G1124  Noniodized Sodium Chloride (Salt, NaCl) G1118  WB Column Coupling (6) 20050  Graduated Cylinder, 50 mL 20250  Graduated Cylinder, 250 mL 20100  Digital Scale with AC Adapter 20200  Commercial Blender with Stainless Steel Container 20700  Wash Bottle, 500 mL 21010  Cuvette Rack

Cat. No. Description 21020 Single Position Pump Stand† 23040  Vortex Mixer 20501  500 mL Bottle Dispenser for Methanol (0-3 mL range) 36020  Filter Funnel, 65 mm (10 per Pack) 36022  Filter Funnel, 105 mm (4 per pack) G4033  Micro-pipettor, 1 mL 20656  Micro-pipette Tips for 1 mL Micro-pipettor (100)   Also required; must be sourced locally: Distilled, Reverse Osmosis, or Deionized Water Ochratoxin Standard 50 ng/μL from Supelco † Multiple position pump stands available * The green coffee procedure for OchraTest WB requires different materials and equipment. Please consult your sales representative for additional information.

Subject to change without notice.
©2006 VICAM. OchraTest WB is a trademark of VICAM.
All VICAM products are protected by worldwide patents and trademarks.

VICAM
313 PLEASANT STREET
WATERTOWN, MA 02472
TEL: 800-338-4381, 617-926-7045
FAX: 617-923-8055
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analyticalmethods presented in this data sheet have been researched and developed by VICAM to be used exclusively with OchraTest WB products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the OchraTest WB procedures.

The user assumes all risk in using OchraTest WB analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that OchraTest WB products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or OchraTest WB products.

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©2001, VICAM