VICAM

• Mycotoxin
• Microbiological

OchraTest Testing System

Ochratoxin A is a mycotoxin produced by the fungus Aspergillus ochraceous and also by several species of Penicillium. Ochratoxin A has been known to cause kidney damage in animals and is considered a carcinogen. Using monoclonal antibody-based affinity chromatography, OchraTest™ from VICAM is the trusted ochratoxin test that produces precise numerical results.

OchraTest is safe and simple. It can be performed in less than 10 minutes and requires no special skills. Results may be recorded using a digital fluorometer readout or automatic printing devices.

OchraTest is also ideal as the cleanup step for any HPLC analysis. Unaffected by heat or humidity, OchraTest has a long shelf life. With OchraTest, you have the best of all worlds: sensitivity, simplicity, and speed—quick tests for parts per billion levels. In fact, no other test comes close for speed, quantification, and economy.

Benefits Convenient - For use with fluorometer or HPLC Durable - Long shelf life; requires no refrigeration Versatile - Can be used with a variety of samples Quick - Less than 10 minutes to isolate toxin* Wide Range - Detects levels as low as 0.25 ppb and as high as 100 ppb Easy - No special skills required, test can be performed virtually anywhere Safe - Requires less toxic materials than other methods *excludes preparation and extraction

Here’s How Simple OchraTest is to Use: Extract Sample Grind and weigh sample Add PEG and water to sample Filter Dilute and Filter Dilute extract Filter Absorb and Elute Pass filtrate over OchraTest affinity column Wash column with buffer and water   Elute ochratoxin and collect in cuvette Measure Place cuvette into a calibrated fluorometer, or Inject eluate into HPLC Determine ochratoxin concentration

Ordering Information

Cat. No. Description G8006 OchraTestTM Basic Equipment Package 110 V G8007  OchraTest Basic Equipment Package 220 V G1021  OchraTest Columns (50 per box) 13012  OchraTest Columns (25 per box) G1105  5x Concentrate of 2% Tween PBS (300mL)* G1112  10x Concentrate of 0.1% Tween PBS (150 mL)** G1113  10x Concentrate PBS Buffer (150 mL) G1114  10x Concentrate of 0.01% Tween PBS (150 mL)* G1124  Noniodized Sodium Chloride (Salt, NaCl) G1130  Acetonitrile (4 x 4 L) G2005  Microfiber Filters, 1.0 μm, 9 cm (100) G4033  Micro-pipettor, 1 mL G8000  Series-4EX Fluorometer G8035  OchraTest Upgrade Pack 20050  Graduated Cylinder, 50 mL 20100  Digital Scale with AC Adapter 20200  Commercial Blender with Stainless Steel Container 20250  Graduated Cylinder, 250 mL 20300  Glass Blender Jar 20501  500 mL Bottle Dispenser for Methanol (0-3 mL range) 20656  Micro-pipette Tips for 1 mL Micropipettor (100) 20657  Disposable Plastic Pipets, 1.5 mL (50 per pack)

Cat. No. Description 20700  Wash Bottle, 500 mL 21010  Cuvette Rack 21020  Single Position Pump Stand† 23040  Vortex Mixer 31240  VICAM Fluted Filter Paper, 24 cm (100) 31955  Microfiber Filters, 1.5 μm, 11 cm (100) 31967  Kim Wipes 32014  Mycotoxin Wash Buffer (200 mL) 32016  OchraTest Eluting Solution (50 mL) 33020  Mycotoxin Calibration Standards 34000  Disposable Cuvettes (250 per pack) 35016  Methanol, HPLC Grade (4 x 4 L) 36010  Disposable Plastic Beakers (25 per pack) 36020  Filter Funnel, 65 mm (10 per pack) 36022  Filter Funnel, 105 mm (4 per pack)   Also required; must be sourced locally: Distilled, Reverse Osmosis, or Deionized Water Sodium Bicarbonate   * Coffee procedures require differentmaterial and equipment. Please consult your sales representative for additional information.   ** Required for testing of currants, figs, and raisins.   †Multiple position pump stands available

Subject to change without notice. ©1998, 2002, 2007 VICAM. OchraTest™ is a trademark of VICAM. All VICAM products are protected by worldwide patents and trademarks.

VICAM
313 PLEASANT STREET
WATERTOWN, MA 02472
TEL: 800-338-4381, 617-926-7045
FAX: 617-923-8055
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with OchraTest™ products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the OchraTest procedures.

The user assumes all risk in using OchraTest analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that OchraTest products conform to VICAM’s printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or OchraTest products..

Back to Top

©2001, VICAM