VICAM

• Mycotoxin
• Microbiological

ZearalaTest WB Testing System

Zearalenone is an estrogenic mycotoxin produced by the fungus Fusarium graminearum. Zearalenone induces feminization in animals at concentrations in feed of about 1 mg/kg. Higher concentrations (50–100 mg/kg) can interfere with conception, ovulation, implantation, fetal development, and the viability of newborn animals. Increasingly concerned with human ingestion of this mycotoxin, the European Union is considering a full slate of proposed regulations for zearalenone in foods ranging from bread to breakfast cereals.

Using monoclonal antibody-based affinity chromatography, ZearalaTest™WB is a new HPLC-only test for the detection of zearalenone using wide bore immunoaffinity columns.With a total volume of 3 mL, compared to the total of 1 mL in VICAM’s standard columns, ZearalaTest WB allows for a faster flow rate preferred by many laboratories and is the ideal cleanup step for any HPLC.

ZearalaTest WB is one of many wide bore columns available from VICAM. Through the development of this fast-flow WB product line,VICAM demonstrates a commitment to improving mycotoxin testing and responding to our customers’needs.

Benefits Exclusive – Specifically for HPLC use Durable – Long shelf life; requires no refrigeration Versatile – For use with a variety of samples Quick –20 minutes to isolate toxin* Wide Range – Detects levels as high as 1500 ng Fast Flow – Passes more volume over the column *excluding preparation and extraction

Here’s How Simple Zearalatest WB is to Use: Extract Sample Grind and weigh sample Add salt and methanol/water mixture Filter Dilute and Filter Dilute extract Filter Absorb and Elute Pass filtrate over ZearalaTest WB affinity column Wash column Elute zearalanone and collect in a cuvette Measure Inject eluate into HPLC Determine zearalanone concentration

Ordering Information

Cat. No. Description G1026 ZearalaTestWB Columns (25 per Box) 31240  VICAM Fluted Filter Paper, 24 cm (100) 31955  Microfiber Filters, 1.5 μm, 11 cm (100) 34000  Disposable Cuvettes (250 per Pack) 35016  Methanol, HPLC Grade (4 X 4 L) G1124  Noniodized Sodium Chloride (Salt,NaCl) G1118  WB Column Coupling (6) 20050  Graduated Cylinder, 50 mL 20100  Digital Scale with AC Adapter 20200  Commercial Blender with Stainless Steel Container 20300  Glass Blender Jar 20250  Graduated Cylinder, 250 mL 20700  Wash Bottle, 500 mL

Cat. No. Description 21010  Cuvette Rack 21020  Single Position Pump Stand† 23040  Vortex Mixer 36020  Filter Funnel, 65 mm (10 per Pack) 36022  Filter Funnel, 105 mm (4 per Pack) 20656  Micro-pipette Tips for 1 mL Micro-pipettor (100) G4033  Micro-pipettor, 1 mL Also required; must be sourced locally: Distilled, Reverse Osmosis, or Deionized Water Zearalenone Standard 50 ng/μL from Supelco † Multiple position pump stands available

Subject to change without notice.
©2006 VICAM. ZearalaTest WB is a trademark of VICAM. All VICAM products are protected by worldwide patents and trademarks.

VICAM
313 PLEASANT STREET
WATERTOWN, MA 02472
TEL: 800-338-4381, 617-926-7045
FAX: 617-923-8055
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with ZearalaTest™WB products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the ZearalaTest WB procedures.

The user assumes all risk in using ZearalaTest WB analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that ZearalaTest WB products conform to VICAM’s printed specification and quality control standards.VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose.VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or ZearalaTest WB products..

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©2001, VICAM