VICAM

• Mycotoxin

ZearalaTest Testing System

Zearalenone is an estrogenic mycotoxin produced by the fungus Fusarium graminearum. Zearalenone induces feminization in animals at concentrations in feed of about 1 ppm. Higher concentrations (50-100 ppm) can interfere with conception, ovulation, implantation, fetal development, and the viability of newborn animals. Using monoclonal antibody-based affinity chromatography, ZearalaTest™ from VICAM is the trusted zearalenone test that produces precise numerical results.

ZearalaTest is safe and simple. It can be performed in less than 15 minutes and requires no special skills. Results may be recorded using a digital fluorometer readout or automatic printing devices. ZearalaTest is also ideal as the cleanup step for any HPLC or TLC analysis.With ZearalaTest you have the best of all worlds: sensitivity, simplicity, and speed—quick tests for parts per million levels. In fact, no other test comes close for speed, quantification, and economy.

Download a Datasheet for this Product (PDF)

Benefits Convenient – For use with fluorometer or HPLC Durable – Long shelf life; requires no refrigeration Versatile – For use with a variety of samples Quick – Less than 15 minutes to isolate toxin* Wide Range – Detects levels as low as 0.1 ppm and as high as 5 ppm Safe – Requires less toxic materials than conventional methods *excluding preparation and extraction

Here’s How Simple Zearalatest is to Use: Extract Sample Grind and weigh sample Add salt andmethanol/water or cetonitrile/watermixture Filter Dilute and filter Dilute extract Filter Absorb and Elute Pass filtrate over ZearalaTest affinity column Wash column Elute zearalenone and collect in cuvette Measure Add Developer and place cuvette into a calibrated fluorometer, or Inject Eluate into HPLC Determine zearalenone concentration

Ordering Information

Cat. No. Description G8010  ZearalaTest Basic Equipment Package (110 Volt) G8036  ZearalaTest Upgrade Pack G1012  ZearalaTest Columns (25 per box) 33020  Mycotoxin Calibration Standards 32011  ZearalaTest Developer (50 mL) G1112  10x concentrate of 0.1% Tween PBS (150 mL) G1124  Noniodized Sodium Chloride (Salt, NaCl) 35016  Methanol, HPLC Grade (4 x 4L) G1130  Acetonitrile (4 x 4L) 34000  Disposable Cuvettes (250 per pack) 21010  Cuvette Rack 31240  VICAM Fluted Filter Paper, 24 cm (100) G2005  Microfiber Filters, 1.0 μm, 9 cm (100) 31967  Kim Wipes 20700  Wash Bottle, 500 mL 20501  500 mL Bottle Dispenser for Methanol (0-3 mL range) 20652  Disposable Plastic Pipets, 1mL (50 per pack)

Cat. No. Description 20656  Micro-pipette tips for 1mL Micro-pipettor G4033  Micro-pipettor, 1 mL 36020  Filter Funnel, 65 mm (10 per pack) 36022  Filter Funnel, 105 mm (4 per pack) 36010  Disposable Plastic Beakers (25 per pack) 20050  Graduated Cylinder, 50 mL 20250  Graduated Cylinder, 250 mL 21020  Single Position Pump Stand† 20100  Digital Scale with AC Adapter 20200  Commercial Blender with Stainless Steel Container 20300  Glass Blender Jar G8000  Series-4EX Fluorometer 23040  Vortex Mixer   Also required; must be sourced locally: Distilled, Reverse Osmosis, or Deionized Water   †Multiple position pumps stands available

Subject to change without notice. ©2006 VICAM. ZearalaTest™ is a trademark of VICAM. All VICAM products are protected by worldwide patents and trademarks.

VICAM, a Waters Business
34 MAPLE ST.
MILFORD, MA 01757
TEL: 800-338-4381, 508-482-4935
FAX: 508-482-4972
Web site: www.vicam.com
e-mail: vicam@vicam.com

The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with ZearalaTest™ products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the ZearalaTest procedures.

The user assumes all risk in using ZearalaTest™ analytical procedures and products. VICAM makes no warranty of any kind, express or implied, other than that ZearalaTest products conform to VICAM’s printed specification and quality control standards. VICAM will at its option repair or replace any product or part thereof which proves to be defective in workmanship or material. VICAM’s undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or ZearalaTest products.

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©2001, VICAM